Anticorps Monoclonal anti-HLA-F

HLA-F Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B12C2

N° de cat : 66819-1-PBS

Synonymes

1B12C2, CDA12, HLA 5.4, HLA CDA12, HLA F

---

Galerie de données de validation

• 

  WB analysis using 66819-1-Ig (same clone as 66819-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66819-1-Ig (same clone as 66819-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66819-1-Ig (same clone as 66819-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66819-1-Ig (same clone as 66819-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 66819-1-Ig (same clone as 66819-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-F antibody (66819-1-Ig, Clone: 1B12C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66819-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66819-1-PBS cible HLA-F dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HLA-F Protéine recombinante Ag6475
• Nom complet: major histocompatibility complex, class I, F
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC062991
• Symbole du gène: HLA-F
• Identification du gène (NCBI): 3134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

{{ptg:RelatedPrimaryAntibodies}}