Anticorps Monoclonal anti-HLA-G

HLA-G Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E5A10

N° de cat : 66447-1-PBS

Synonymes

HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G

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Galerie de données de validation

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  WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  Human placenta tissue and A375 cell lines (negtive control) were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66447-1-PBS cible HLA-G dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HLA-G Protéine recombinante Ag10839
• Nom complet: major histocompatibility complex, class I, G
• Masse moléculaire calculée: 338 aa, 38 kDa
• Poids moléculaire observé: 33-45 kDa
• Numéro d’acquisition GenBank: BC021708
• Symbole du gène: HLA-G
• Identification du gène (NCBI): 3135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). HLA-G is a non-classical MHC class I molecule with multiple immunoregulatory properties. HLA-G exhibits a restricted pattern of expression that includes placental extravillous trophoblasts at the maternal-fetal interface, where it abolishes maternal immune cell activity against fetus and establishes immune tolerance. Aberrant expression of HLA-G has been found in a variety of human neoplastic diseases. It plays an important role in the escape of tumor cells from immunosurveillance.

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