Anticorps Polyclonal de lapin anti-HLF

HLF Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12870-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12870-1-AP (same clone as 12870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12870-1-AP (same clone as 12870-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12870-1-AP (same clone as 12870-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12870-1-AP (same clone as 12870-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12870-1-AP (same clone as 12870-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12870-1-PBS cible HLF dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLF Protéine recombinante Ag3533
• Nom complet: hepatic leukemia factor
• Masse moléculaire calculée: 295 aa, 33 kDa
• Poids moléculaire observé: 33kDa, 66 kDa
• Numéro d’acquisition GenBank: BC036093
• Symbole du gène: HLF
• Identification du gène (NCBI): 3131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hepatic leukemia factor (Hlf), is a novel hepatic protein of the proline and acidic amino acid-rich basic leucine zipper (bZip) family, previously studied for its role in circadian rhythm regulated neurotransmitter metabolism in the brain (PMID: 29166614). HLF is an essential regulator of HSC quiescence and that HLF deficiency leads to an exhaustion of HSCs in adult mouse BM during regeneration or following cytotoxic stress (PMID: 29262330).Hlf is able to bind DNA specifically as a homodimer or as a heterodimer with other PAR factors (PMID: 1516826). Western blot analysis detected bands  33 kDa whereas the 66 kDa could be the dimer form of Hlf.

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