Anticorps Recombinant de lapin anti-HLTF

HLTF Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251000A4

N° de cat : 86362-2-RR

Synonymes

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC:2.3.2.27, Helicase-like transcription factor, HIP116, HIP116A

---

Galerie de données de validation

• 

  WB analysis using 86362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86362-2-RR (HLTF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86362-2-RR (HLTF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86362-2-RR against Human HLTF were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules U-937, cellules COS-7, cellules HCT 116, cellules HeLa, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86362-2-RR cible HLTF dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HLTF Protéine recombinante Ag5596
• Nom complet: helicase-like transcription factor
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC044659
• Symbole du gène: HLTF
• Identification du gène (NCBI): 6596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Helicase-like Transcription Factor (HLTF) is a crucial nuclear protein and member of the SWI/SNF family of chromatin-remodeling complexes, playing a dual role in transcription regulation and DNA damage repair. It functions dually as a regulator of gene expression, utilizing its ATP-dependent helicase activity to modify chromatin structure and facilitate transcription, and as a central effector in the DNA damage response pathway. HLTF is particularly crucial for promoting error-free DNA repair at stalled replication forks via a mechanism called template switching, which prevents the accumulation of mutagenic lesions. HLTF can be detected as 114 kDa.

{{ptg:RelatedPrimaryAntibodies}}