Anticorps Polyclonal de lapin anti-HLX

• Phare
• Validé par KD/KO

HLX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14336-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human placenta using 14336-1-AP (same clone as 14336-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14336-1-AP (HLX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14336-1-AP (same clone as 14336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 14336-1-AP (same clone as 14336-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14336-1-AP (HLX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 14336-1-AP (same clone as 14336-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14336-1-AP (HLX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14336-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14336-1-PBS cible HLX dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLX Protéine recombinante Ag5627
• Nom complet: H2.0-like homeobox
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC033808
• Symbole du gène: HLX
• Identification du gène (NCBI): 3142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

In immune system, Hlx is a Th1-special transcription factor that interacts specifically with T-bet, and synergistically they promote IFN-γ expression when co-expressed. It can down-regulate the IL-4 receptor expression in naive CD4+ T cells [PMID:14688316]. Over expression of Hlx contributes ot the aberrant expression of IFN-γ in normal CD4+ T cells during the differentiation under Th2-polarizing conditions. In addition, Hlx is a negative regulator of IFN-γ production in NK cells and its inhibitory function is achieved at least in part through the proteasomal degradation of STAT4 [PMID:16424184].

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