Anticorps Polyclonal de lapin anti-HMBOX1

• Phare
• Validé par KD/KO

HMBOX1 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16123-1-AP

Synonymes

HMBOX1, HNF1LA, homeobox containing 1, Homeobox containing protein 1, PBHNF

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16123-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16123-1-AP (HMBOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16123-1-AP

  IP Result of anti-HMBOX1 (IP:16123-1-AP, 4ug; Detection:16123-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

16123-1-AP cible HMBOX1 dans les applications de WB, IP, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMBOX1 Protéine recombinante Ag9081
• Nom complet: homeobox containing 1
• Masse moléculaire calculée: 420 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC009259
• Symbole du gène: HMBOX1
• Identification du gène (NCBI): 79618
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	ChIP	Mol Ther; Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.; Authors - Chenfei Zhou ; KD Validated
human,rat	WB	Front Cardiovasc Med; Exosome-Derived From Sepsis Patients' Blood Promoted Pyroptosis of Cardiomyocytes by Regulating miR-885-5p/HMBOX1.; Authors - Guo-Wei Tu
		Oncol Rep; Knockdown of homeobox containing 1 increases the radiosensitivity of cervical cancer cells through telomere shortening.; Authors - Shuliang Zhou
human	WB	Exp Ther Med; HMBOX1 attenuates LPS-induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF-κB signaling pathway.; Authors - Minyuan Nie
human	WB,IHC	Cell Death Dis; WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m6A-dependent manner.; Authors - Shijie Chen ; KD Validated
human	WB	J Cell Mol Med; 5,2'-dibromo-2,4',5'-trihydroxydiphenylmethanone attenuates LPS-induced inflammation and ROS production in EA.hy926 cells via HMBOX1 induction.; Authors - Hong-Xia Yuan