Anticorps Polyclonal de lapin anti-HMCN1

HMCN1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18837-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse skin using 18837-1-AP (same clone as 18837-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 18837-1-AP (HMCN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18837-1-AP (same clone as 18837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18837-1-AP (same clone as 18837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 18837-1-AP (same clone as 18837-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18837-1-AP (same clone as 18837-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18837-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18837-1-PBS cible HMCN1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: hemicentin 1
• Masse moléculaire calculée: 613 kDa, 600 kDa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_031935
• Symbole du gène: HMCN1
• Identification du gène (NCBI): 83872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.

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