Anticorps Monoclonal anti-HMG20B

HMG20B Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4C2A9

N° de cat : 67354-1-PBS

Synonymes

4C2A9, BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35

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Galerie de données de validation

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  WB analysis using 67354-1-Ig (same clone as 67354-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67354-1-Ig (same clone as 67354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67354-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67354-1-PBS cible HMG20B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HMG20B Protéine recombinante Ag6103
• Nom complet: high-mobility group 20B
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC002552
• Symbole du gène: HMG20B
• Identification du gène (NCBI): 10362
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation (PMID:10449787). The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse (PMID:15240883). HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure (PMID:19997604). The predicted molecular weight of the HMG20B protein is 36 kDa, while the molecular weight detected in the literature is approximately 40 kDa by SDS-PAGE (PMID: 36171271).

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