Anticorps Polyclonal de lapin anti-HMGA1

HMGA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 29895-1-AP

Synonymes

HMG-I(Y), HMGA1A, High mobility group protein R, High mobility group protein HMG-I/HMG-Y, High mobility group protein A1

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Galerie de données de validation

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  WB analysis using 29895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29895-1-AP (HMGA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 29895-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 29895-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using HMGA1 antibody (29895-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MKN-45
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

29895-1-AP cible HMGA1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGA1 Protéine recombinante Ag31866
• Nom complet: high mobility group AT-hook 1
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC071864
• Symbole du gène: HMGA1
• Identification du gène (NCBI): 3159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).

Publications

Species	Application	Title
human	IHC	Sci Rep; A method for the identification of lactate metabolism-related prognostic biomarkers and its validations in non-small cell lung cancer; Authors - Weiyang Yang
human	WB	Pathol Oncol Res; Long non-coding RNA MYU promotes ovarian cancer cell proliferation by sponging miR-6827-5p and upregulating HMGA1; Authors - Shaoyu Wang
human	IHC	J Thorac Dis; Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients; Authors - Zhanming Ma
human	WB	Chem Sci; A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation; Authors - Yi-Cheng Sin
human	WB	EPMA J; Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies; Authors - Jinjin Zhang

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