Anticorps Polyclonal de lapin anti-HMGA2

• Phare
• Validé par KD/KO

HMGA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20795-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20795-1-AP (same clone as 20795-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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  WB analysis using 20795-1-AP (same clone as 20795-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 20795-1-AP (same clone as 20795-1-PBS)

  IP result of anti-HMGA2 (IP:20795-1-AP, 4ug; Detection:20795-1-AP 1:1000) with NIH/3T3 cells lysate 3200ug. This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 20795-1-AP (same clone as 20795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 20795-1-AP (same clone as 20795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20795-1-AP (same clone as 20795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20795-1-AP (same clone as 20795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 20795-1-AP (same clone as 20795-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HMGA2 antibody (20795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20795-1-PBS cible HMGA2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGA2 Protéine recombinante Ag14588
• Nom complet: high mobility group AT-hook 2
• Masse moléculaire calculée: 108 aa, 12 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: NM_003483
• Symbole du gène: HMGA2
• Identification du gène (NCBI): 8091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (PMID: 18202751,19551524). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (18425117). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (19551524). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (PMID: 20228781). The calcualted molecular weight of HMGA2 is 12 kDa, but modified HMGA2 is about 18-20 kDa. (PMID: 18505920 )

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