Anticorps Monoclonal anti-HMGB1

HMGB1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D12A6

N° de cat : 66525-1-PBS

Synonymes

HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1, high mobility group box 1

---

Galerie de données de validation

• 

  WB analysis using 66525-1-Ig (same clone as 66525-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66525-1-Ig (same clone as 66525-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66525-1-Ig (same clone as 66525-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66525-1-Ig (same clone as 66525-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66525-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66525-1-PBS cible HMGB1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HMGB1 Protéine recombinante Ag1264
• Nom complet: high-mobility group box 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003378
• Symbole du gène: HMGB1
• Identification du gène (NCBI): 3146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

{{ptg:RelatedPrimaryAntibodies}}