Anticorps Recombinant de lapin anti-HMGB1

HMGB1 Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230182C11

N° de cat : 82973-2-PBS

Synonymes

230182C11, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1

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Galerie de données de validation

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  WB analysis using 82973-2-RR (same clone as 82973-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82973-2-RR (HMGB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82973-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00311-2

  Sandwich ELISA standard curve of MP00311-2, HMGB1 Recombinant Matched Antibody Pair, PBS Only. 82973-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1264. 82973-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  FC experiment of Jurkat using 82973-2-RR (same clone as 82973-2-PBS)

  (blue)1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human HMGB1 (82973-2-RR, Clone:230182C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82973-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82973-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 82973-2-PBS against Human HMGB1 was performed. The affinity constant is 2.48 nM.

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Informations sur le produit

82973-2-PBS cible HMGB1 dans les applications de Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HMGB1 Protéine recombinante Ag1264
• Nom complet: high-mobility group box 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC003378
• Symbole du gène: HMGB1
• Identification du gène (NCBI): 3146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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