Anticorps Polyclonal de lapin anti-HMGB2

• Phare
• Validé par KD/KO

HMGB2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 14597-1-AP

Synonymes

high mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG2, HMGB2

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Galerie de données de validation

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  WB analysis of Jurkat using 14597-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14597-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14597-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 14597-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14597-1-AP (HMGB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14597-1-AP

  IP Result of anti-HMGB2 (IP:14597-1-AP, 4ug; Detection:14597-1-AP 1:500) with HEK-293 cells lysate 1200ug.

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  IHC staining of mouse brain using 14597-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14597-1-AP (HMGB2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14597-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14597-1-AP (HMGB2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14597-1-AP (HMGB2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HL-60, cellules K-562, tissu de cervelet humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

14597-1-AP cible HMGB2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGB2 Protéine recombinante Ag6135
• Nom complet: high-mobility group box 2
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC001063
• Symbole du gène: HMGB2
• Identification du gène (NCBI): 3148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

High mobility group protein B2 (HMGB2) belongs to a family of highly conserved proteins that contain HMG box domains (11246022,14871457). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein (14871457). HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals (11262228). HMGB3 is only expressed during embryogenesis (9598312). While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix . HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters (11246022,14871457). Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes (19317908 ,10490593). In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation endproducts (RAGE) (19811285 ). Research studies have shown that HMGB2 overexpression in hepatocellular carcinoma is associated with poor prognosis and shorter survival time (20851854).This antibody recognizes the phosphorylation form of HMGB2 protein. The calculated molecular weight of HMGB2 is 24 kDa, but the post-modifiction of HMGB2 is about 33-35 kDa. (PMID: 18218727)

Publications

Species	Application	Title
rat	WB	Environ Pollut; Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.; Authors - Yongli Han
mouse	WB	Chemosphere; Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.; Authors - Yongli Han
human	WB	Cancer Gene Ther; The LncRNA RP11-301G19.1/miR-582-5p/HMGB2 axis modulates the proliferation and apoptosis of multiple myeloma cancer cells via the PI3K/AKT signalling pathway.; Authors - Faming Wang
human	WB	Cell Commun Signal; HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer.; Authors - Deyuan Fu ; KD Validated
mouse		Sci Rep; Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.; Authors - Zhiping Cao
human	WB	Eur J Pharmacol; LncRNA SNHG16 accelerates atherosclerosis and promotes ox-LDL-induced VSMC growth via the miRNA-22-3p/HMGB2 axis; Authors - Yiyong Wang ; KD Validated