Anticorps Recombinant de lapin anti-HMGB2

• Phare
• Validé par KD/KO

HMGB2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240453H5

N° de cat : 83482-5-PBS

Synonymes

240453H5, high mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 83482-5-RR (same clone as 83482-5-PBS)

  WB result of HMGB2 antibody (83482-5-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMGB2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83482-5-RR (same clone as 83482-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGB2 antibody (83482-5-RR, Clone: 240453H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83482-5-RR (same clone as 83482-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB2 Recombinant antibody (83482-5-RR, Clone:240453H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83482-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83482-5-RR against Human HMGB2 were performed. The affinity constant is 44.4 nM.

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Informations sur le produit

83482-5-PBS cible HMGB2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HMGB2 Protéine recombinante Ag6135
• Nom complet: high-mobility group box 2
• Masse moléculaire calculée: 24 kDa
• Numéro d’acquisition GenBank: BC001063
• Symbole du gène: HMGB2
• Identification du gène (NCBI): 3148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

High mobility group protein B2 (HMGB2) belongs to a family of highly conserved proteins that contain HMG box domains (11246022,14871457). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein (14871457). HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals (11262228). HMGB3 is only expressed during embryogenesis (9598312). While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix . HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters (11246022,14871457). Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes (19317908 ,10490593). In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation endproducts (RAGE) (19811285 ). Research studies have shown that HMGB2 overexpression in hepatocellular carcinoma is associated with poor prognosis and shorter survival time (20851854).The calculated molecular weight of HMGB2 is 24 kDa, and the post-modifiction of HMGB2 is about 33-35 kDa. (18218727)

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