Anticorps Recombinant de lapin anti-HMGCL

HMGCL Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242224G10

N° de cat : 84769-1-PBS

Synonymes

242224G10, EC:4.1.3.4, HL, HMG CoA lyase, HMG-CoA lyase

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Galerie de données de validation

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  WB analysis using 84769-1-RR (same clone as 84769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84769-1-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84769-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01555-2

  Cytometric bead array standard curve of MP01555-2, HMGCL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84769-2-PBS. Detection antibody: 84769-1-PBS. Standard: Ag10426. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84769-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84769-1-RR against Human HMGCL were performed. The affinity constant is 0.408 nM.

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Informations sur le produit

84769-1-PBS cible HMGCL dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HMGCL Protéine recombinante Ag10426
• Nom complet: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Masse moléculaire calculée: 325 aa, 34 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC010570
• Symbole du gène: HMGCL
• Identification du gène (NCBI): 3155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

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