Anticorps Recombinant de lapin anti-HMGCL

HMGCL Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242224F9

N° de cat : 84769-4-PBS

Synonymes

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9

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Galerie de données de validation

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  WB analysis using 84769-4-RR (same clone as 84769-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84769-4-RR (same clone as 84769-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84769-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84769-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

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Informations sur le produit

84769-4-PBS cible HMGCL dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HMGCL Protéine recombinante Ag10426
• Nom complet: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
• Masse moléculaire calculée: 325 aa, 34 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC010570
• Symbole du gène: HMGCL
• Identification du gène (NCBI): 3155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

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