Anticorps Polyclonal de lapin anti-HMGCS1

• Phare
• Validé par KD/KO

HMGCS1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (3)

N° de cat : 17643-1-AP

Synonymes

HMG CoA synthase, HMGCS, HMGCS1

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Galerie de données de validation

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  WB analysis of HepG2 using 17643-1-AP

  WB result of HMGCS1 antibody (17643-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMGCS1 transfected HepG2 cells.

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  WB analysis using 17643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 17643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17643-1-AP

  IP Result of anti-HMGCS1 (IP:17643-1-AP, 3ug; Detection:17643-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IF Staining of HepG2 using 17643-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HMGCS1 antibody (17643-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HepG2, cellules LNCaP, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 28 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

17643-1-AP cible HMGCS1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGCS1 Protéine recombinante Ag11825
• Nom complet: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
• Masse moléculaire calculée: 520 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC083514
• Symbole du gène: HMGCS1
• Identification du gène (NCBI): 3157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).

Publications

Species	Application	Title
human	WB	Cancer Cell; Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis; Authors - Wei Zhou ; KD Validated
human	WB	Nat Cancer; Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism; Authors - Linjie Zhao ; KD Validated
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse,human	IF,WB	J Cell Biol; TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.; Authors - Wan Yun Ho
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
human	WB	Mol Cell Proteomics; Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.; Authors - Saraon Punit P

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K (Verified Customer) (12-20-2020)

Used for western blotting (1:1000) and IHC (1:200), worked well for me

• Applications: Western Blot
• Cell Tissue Type: mouse liver tissues

FH

H (Verified Customer) (07-09-2020)

This antibody is truly good. No non-specific band detected. The target band is very strong when 15ug of cell lysate was loaded.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and HCT116

HMGCS1 Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:17643-1-AP)

FH

Boyan (Verified Customer) (07-08-2019)

This antibody recognises a clean, and clear band at the expected molecular weight. It goes down when you treat the cells with cholesterol.

• Applications: Western Blot,
• Cell Tissue Type: MEF