Anticorps Polyclonal de lapin anti-HMGN2

HMGN2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10953-1-PBS

Synonymes

Non-histone chromosomal protein HMG-17, HMG17, High mobility group nucleosome-binding domain-containing protein 2

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Galerie de données de validation

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  WB analysis of Jurkat using 10953-1-AP (same clone as 10953-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10953-1-AP (same clone as 10953-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10953-1-AP (same clone as 10953-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10953-1-AP (same clone as 10953-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10953-1-AP (same clone as 10953-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10953-1-AP (same clone as 10953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10953-1-AP (same clone as 10953-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGN2 antibody (10953-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10953-1-PBS cible HMGN2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGN2 Protéine recombinante Ag1401
• Nom complet: high-mobility group nucleosomal binding domain 2
• Masse moléculaire calculée: 9 aa, 3 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC014644
• Symbole du gène: HMGN2
• Identification du gène (NCBI): 3151
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.

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