Anticorps Polyclonal de lapin anti-HMHA1

HMHA1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14832-1-AP

Synonymes

HA 1, HLA HA1, HMHA1, KIAA0223, mHag HA 1

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Galerie de données de validation

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  WB analysis using 14832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14832-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14832-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14832-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsil using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsil using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14832-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa, cellules Jurkat, cellules K-562, tissu de thymus de souris
• Résultats positifs en IHC: tissu amygdalien humain, tissu splénique de souris, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

14832-1-AP cible HMHA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMHA1 Protéine recombinante Ag6636
• Nom complet: histocompatibility (minor) HA-1
• Masse moléculaire calculée: 1136 aa, 125 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC065223
• Symbole du gène: HMHA1
• Identification du gène (NCBI): 23526
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Aging Dis; Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke; Authors - Hongyu Ma