- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-HMMR-Specific
HMMR-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 15820-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HepG2, cellules C6, cellules K-562, cellules T-47D |
| Résultats positifs en IHC | tissu d'amygdalite humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules HepG2, |
| Résultats positifs en FC (Intra) | cellules HepG2 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
15820-1-AP cible HMMR-Specific dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | hyaluronan-mediated motility receptor (RHAMM) |
| Masse moléculaire calculée | 84 kDa |
| Poids moléculaire observé | 84 kDa |
| Numéro d’acquisition GenBank | NM_001142556 |
| Symbole du gène | HMMR |
| Identification du gène (NCBI) | 3161 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85 kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for HMMR-Specific antibody 15820-1-AP | Download protocol |
| IHC protocol for HMMR-Specific antibody 15820-1-AP | Download protocol |
| IF protocol for HMMR-Specific antibody 15820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Transl Med Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance | ||
Int J Mol Sci Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat | ||
Int J Biol Sci Oleate acid-stimulated HMMR expression by CEBPα is associated with nonalcoholic steatohepatitis and hepatocellular carcinoma.
| ||
J Cell Physiol A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells. | ||
Acta Biochim Biophys Sin (Shanghai) FOXM1 mediates methotrexate resistance in osteosarcoma cells by promoting autophagy |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Heriberto (Verified Customer) (11-28-2018) | Observed multiple bands at different molecular weights. Was not specific for the detection of HMMR .
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