Anticorps Polyclonal de lapin anti-HMOX2

• Phare
• Validé par KD/KO

HMOX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 14817-1-AP

Synonymes

EC:1.14.14.18, heme oxygenase (decycling) 2, Heme oxygenase 2, Heme oxygenase 2 soluble form, HO 2

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Galerie de données de validation

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  WB analysis of HepG2 using 14817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 14817-1-AP

  WB result of HMOX2 antibody (14817-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMOX2 transfected hTERT-RPE1 cells.

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  WB analysis of Raji using 14817-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14817-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14817-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HepG2 using 14817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14817-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14817-1-AP (HMOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14817-1-AP

  IP result of anti-HMOX2 (IP:14817-1-AP, 4ug; Detection:14817-1-AP 1:500) with Raji cells lysate 2000ug.

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  IHC staining of human lymphoma using 14817-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14817-1-AP (HMOX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14817-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14817-1-AP (HMOX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14817-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HMOX2 antibody (14817-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 14817-1-AP

  Immunofluorescent analysis of Hela cells, using HMOX2 antibody 14817-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules hTERT-RPE1, cellules K-562, cellules Raji, tissu splénique de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

14817-1-AP cible HMOX2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMOX2 Protéine recombinante Ag6575
• Nom complet: heme oxygenase (decycling) 2
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002396
• Symbole du gène: HMOX2
• Identification du gène (NCBI): 3163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Publications

Species	Application	Title
human	WB	Mol Cell; Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.; Authors - Gokhan Unlu
rat	WB	Acta Pharm Sin B; Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.; Authors - Dominik Bakalarz
human	WB	J Cell Sci; Role of epithelial sodium channels (ENaCs) in endothelial function.; Authors - Dongqing Guo
mouse,human	WB,IHC	Oxid Med Cell Longev; Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion.; Authors - László Potor
mouse	WB,IHC	Am J Physiol Gastrointest Liver Physiol; Carbon monoxide produced by HO-1 upregulation is the main factor behind the abnormal motility seen in experimental ulcerative colitis in mice; Authors - Mengchao Zhao
rat	WB	Cells; Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions.; Authors - Katarzyna Magierowska

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