Anticorps Polyclonal de lapin anti-HN1L

HN1L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16285-1-PBS

Synonymes

C16orf34, Hematological and neurological expressed 1-like protein, HN1-like protein, JPT2, Jupiter microtubule associated homolog 2

---

Galerie de données de validation

• 

  WB analysis using 16285-1-AP (same clone as 16285-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16285-1-AP (HN1L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16285-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 16285-1-AP (same clone as 16285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16285-1-AP (HN1L antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16285-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 16285-1-AP (same clone as 16285-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HN1L antibody (16285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16285-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

16285-1-PBS cible HN1L dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HN1L Protéine recombinante Ag9400
• Nom complet: hematological and neurological expressed 1-like
• Masse moléculaire calculée: 190 aa, 20 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC014438
• Symbole du gène: HN1L
• Identification du gène (NCBI): 90861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hematological and neurological expressed 1-like protein (HN1L), also known as Jupiter microtubule-associated homolog 2 (JPT2), belongs to the jupiter family. HN1L is nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061). HN1L enables NAADP to activate Ca2+ release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). HN1L was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal systemHN1L is expressed in liver, kidney, prostate, testis and uterus (PMID: 15094197).

{{ptg:RelatedPrimaryAntibodies}}