Anticorps Polyclonal de lapin anti-HNF1A

• Phare
• Validé par KD/KO

HNF1A Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 22426-1-AP

Synonymes

HNF 1 alpha, HNF 1A, HNF1, HNF1 homeobox A, HNF1A, Hnf1α, LFB1, MODY3, TCF 1, TCF1, Transcription factor 1

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Galerie de données de validation

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  WB analysis of rat liver using 22426-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22426-1-AP (HNF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 22426-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 22426-1-AP (HNF1A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 22426-1-AP

  IP Result of anti-HNF1A (IP:22426-1-AP, 4ug; Detection:22426-1-AP 1:800) with mouse liver tissue lysate 3600ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules SMMC-7721
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 2 publications below

Informations sur le produit

22426-1-AP cible HNF1A dans les applications de WB, IP, IHC, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNF1A Protéine recombinante Ag18188
• Nom complet: HNF1 homeobox A
• Masse moléculaire calculée: 631 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC104910
• Symbole du gène: HNF1A
• Identification du gène (NCBI): 6927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Publications

Species	Application	Title
human	WB	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
human	ChIP	Mol Ther Nucleic Acids; The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2.; Authors - Guifang Du
rat	WB	Acta Pharmacol Sin; Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats.; Authors - Zhen-Li Su
human	WB	Chem Biol Interact; 6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.; Authors - Yuanhang Ma ; KD Validated
human	WB,IHC,CoIP	J Exp Clin Cancer Res; ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.; Authors - Shuohui Dong
	CoIP	Cell Death Dis; CBP/p300 HAT maintains the gene network critical for β cell identity and functional maturity.; Authors - Linlin Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (07-05-2023)

Works with 1X TBST for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Liver tissue