Anticorps Polyclonal de lapin anti-HNF1B

• Phare
• Validé par KD/KO

HNF1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12533-1-PBS

Synonymes

TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta

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Galerie de données de validation

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  WB analysis of L02 using 12533-1-AP (same clone as 12533-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 12533-1-AP (same clone as 12533-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12533-1-AP (same clone as 12533-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 12533-1-AP (same clone as 12533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12533-1-PBS cible HNF1B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNF1B Protéine recombinante Ag3232
• Nom complet: HNF1 homeobox B
• Masse moléculaire calculée: 557 aa, 61 kDa
• Poids moléculaire observé: 58-65 kDa
• Numéro d’acquisition GenBank: BC017714
• Symbole du gène: HNF1B
• Identification du gène (NCBI): 6928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.

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