Anticorps Recombinant de lapin anti-HNF1B

HNF1B Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242120H8

N° de cat : 84684-5-RR

Synonymes

TCF2, HNF-1-beta, HNF-1B, HNF 1 beta, Hepatocyte nuclear factor 1-beta

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Galerie de données de validation

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  IF Staining of HepG2 using 84684-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of Caco-2 using 84684-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HNF1B antibody (84684-5-RR, Clone: 242120H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Caco-2 using 84684-5-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug HNF1B Recombinant antibody (84684-5-RR, Clone:242120H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84684-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84684-5-RR against Human HNF1B were performed. The affinity constant is 13.5 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, cellules Caco-2
• Résultats positifs en FC (Intra): cellules Caco-2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84684-5-RR cible HNF1B dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HNF1B Protéine recombinante Ag3232
• Nom complet: HNF1 homeobox B
• Masse moléculaire calculée: 557 aa, 61 kDa
• Numéro d’acquisition GenBank: BC017714
• Symbole du gène: HNF1B
• Identification du gène (NCBI): 6928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis.

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