Anticorps Polyclonal de lapin anti-HNF4A

HNF4A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 26245-1-PBS

Synonymes

HNF4a9, HNF4a8, HNF4a7, HNF4, HNF 4 alpha

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Galerie de données de validation

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  WB analysis using 26245-1-AP (same clone as 26245-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26245-1-AP (HNF4A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26245-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26245-1-AP (same clone as 26245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 26245-1-AP (HNF4A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26245-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26245-1-AP (same clone as 26245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 26245-1-AP (HNF4A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26245-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26245-1-PBS cible HNF4A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNF4A Protéine recombinante Ag24637
• Nom complet: hepatocyte nuclear factor 4, alpha
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC137539
• Symbole du gène: HNF4A
• Identification du gène (NCBI): 3172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).

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