Anticorps Recombinant de lapin anti-HNF4A

HNF4A Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241593A2

N° de cat : 84316-7-PBS

Synonymes

241593A2, Hepatocyte nuclear factor 4-alpha, HNF 4 alpha, HNF4, HNF4a7

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Galerie de données de validation

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  IHC staining of mouse liver using 84316-7-RR (same clone as 84316-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84316-7-RR (HNF4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84316-7-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84316-7-RR (same clone as 84316-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNF4A antibody (84316-7-RR, Clone: 241593A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84316-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84316-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84316-7-RR against Human HNF4A were performed. The affinity constant is 0.275 nM.

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Informations sur le produit

84316-7-PBS cible HNF4A dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HNF4A Protéine recombinante Ag24647
• Nom complet: hepatocyte nuclear factor 4, alpha
• Masse moléculaire calculée: 53 kDa
• Numéro d’acquisition GenBank: BC137539
• Symbole du gène: HNF4A
• Identification du gène (NCBI): 3172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962).

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