Anticorps Polyclonal de lapin anti-HNRNPA1

• Phare
• Validé par KD/KO

HNRNPA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

33

Avis (2)

N° de cat : 11176-1-AP

Synonymes

Helix destabilizing protein, hnRNP A1, hnRNP core protein A1, HNRNPA1, HNRPA1

---

Galerie de données de validation

• 

  WB analysis using 11176-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11176-1-AP (HNRNPA1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11176-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11176-1-AP (HNRNPA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11176-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA1 antibody (11176-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules K-562, cellules Neuro-2a, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 30 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Informations sur le produit

11176-1-AP cible HNRNPA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPA1 Protéine recombinante Ag1656
• Nom complet: heterogeneous nuclear ribonucleoprotein A1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34, 40 kDa
• Numéro d’acquisition GenBank: BC009600
• Symbole du gène: HNRNPA1
• Identification du gène (NCBI): 3178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Mol Cell; A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.; Authors - Jin-Zhou Huang ; KD Validated
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li ; KD Validated
pig	WB	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
human	WB,IF	J Cell Biol; An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.; Authors - Joseph R Tran

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Zhongwen (Verified Customer) (09-25-2023)

Good quality. It is easy for you to find the target bind.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

HNRNPA1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11176-1-AP)

FH

Katie (Verified Customer) (04-08-2022)

Works nicely for IF in primary CD4+ T cells. 2hr Primary incubation at 37 and 2hr secondary incubation at 37oc 1:1000.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50