Anticorps Polyclonal de lapin anti-HNRNPA2B1

• Phare
• Validé par KD/KO

HNRNPA2B1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(71)

N° de cat : 14813-1-AP

Synonymes

FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPB1, HNRPA2

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Galerie de données de validation

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  WB analysis using 14813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14813-1-AP

  WB result of HNRNPA2B1 antibody (14813-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPA2B1 transfected A549 cells.

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  WB analysis using 14813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14813-1-AP (HNRNPA2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14813-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14813-1-AP (HNRNPA2B1 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HeLa, cellules HL-60, cellules HSC-T6, cellules K-562
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 54 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below

Informations sur le produit

14813-1-AP cible HNRNPA2B1 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPA2B1 Protéine recombinante Ag6563
• Nom complet: heterogeneous nuclear ribonucleoprotein A2/B1
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 36 kDa and 38 kDa
• Numéro d’acquisition GenBank: BC000506
• Symbole du gène: HNRNPA2B1
• Identification du gène (NCBI): 3181
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu
human	IF	Mol Cancer; The therapeutically actionable long non-coding RNA 'T-RECS' is essential to cancer cells' survival in NRAS/MAPK-driven melanoma; Authors - Valentin Feichtenschlager
mouse	WB	Cell Mol Immunol; Inhibition of METTL3 in macrophages provides protection against intestinal inflammation; Authors - Huilong Yin
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB,IF	Mol Cancer; CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner; Authors - Xin Liu
human	WB,IF,CoIP	Theranostics; LINC00982-encoded protein PRDM16-DT regulates CHEK2 splicing to suppress colorectal cancer metastasis and chemoresistance; Authors - Hui-Fang Hu ; KD Validated

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