Anticorps Monoclonal anti-HNRNPA2B1

• Phare
• Validé par KD/KO

HNRNPA2B1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

3H6F7

N° de cat : 67445-1-Ig

Synonymes

DKFZp779B0244, FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1

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Galerie de données de validation

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  WB analysis using 67445-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67445-1-Ig

  WB result of HNRNPA2B1 antibody (67445-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPA2B1 transfected A549 cells.

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  WB analysis using 67445-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67445-1-Ig (HNRNPA2B1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67445-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HNRNPA2B1 antibody (67445-1-Ig, Clone: 3H6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of U2OS using 67445-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HNRNPA2B1 antibody (67445-1-Ig, Clone: 3H6F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:8000-1:32000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

67445-1-Ig cible HNRNPA2B1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HNRNPA2B1 Protéine recombinante Ag6699
• Nom complet: heterogeneous nuclear ribonucleoprotein A2/B1
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000506
• Symbole du gène: HNRNPA2B1
• Identification du gène (NCBI): 3181
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HnRNPA2B1 is one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA and participate in carcinogenesis. It has a role in packaging nascent mRNA, alternative splicing, tycoplamic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport.

Publications

Species	Application	Title
human	WB,IHC	J Transl Med; HNRNPA2B1-mediated m6A modification of lncRNA MEG3 facilitates tumorigenesis and metastasis of non-small cell lung cancer by regulating miR-21-5p/PTEN axis; Authors - Ke Li ; KD Validated
human	WB	Cancer Lett; HNRNPA2B1 regulates tamoxifen- and fulvestrant-sensitivity and hallmarks of endocrine resistance in breast cancer cells.; Authors - Belinda J Petri
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated