Anticorps Recombinant de lapin anti-HNRNPAB

• Phare
• Validé par KD/KO

HNRNPAB Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241462B1

N° de cat : 84207-1-PBS

Synonymes

241462B1, ABBP 1, ABBP1, ABBP-1, APOBEC1-binding protein 1

---

Galerie de données de validation

• 

  WB analysis using 84207-1-RR (same clone as 84207-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 84207-1-RR (same clone as 84207-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 84207-1-RR (same clone as 84207-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01139-1

  Cytometric bead array standard curve of MP01139-1, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-2-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP01139-2

  Cytometric bead array standard curve of MP01139-2, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-3-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84207-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84207-1-PBS against Human HNRNPAB was performed. The affinity constant is 2.01 nM.

• 

  Affinity and Kinetic Characterization of 84207-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

---

Informations sur le produit

84207-1-PBS cible HNRNPAB dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HNRNPAB Protéine recombinante Ag35010
• Nom complet: heterogeneous nuclear ribonucleoprotein A/B
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36-42 kDa
• Numéro d’acquisition GenBank: BC036708
• Symbole du gène: HNRNPAB
• Identification du gène (NCBI): 3182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).

{{ptg:RelatedPrimaryAntibodies}}