Anticorps Recombinant de lapin anti-HNRNPAB

• Phare
• Validé par KD/KO

HNRNPAB Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241462B1

N° de cat : 84207-1-RR

Synonymes

hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1, ABBP 1

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Galerie de données de validation

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  WB analysis using 84207-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 84207-1-RR

  WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells.

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  IF Staining of HeLa using 84207-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84207-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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  FC experiment of Jurkat using 84207-1-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of HeLa using 84207-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84207-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293T, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Jurkat, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84207-1-RR cible HNRNPAB dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HNRNPAB Protéine recombinante Ag35010
• Nom complet: heterogeneous nuclear ribonucleoprotein A/B
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36-42 kDa
• Numéro d’acquisition GenBank: BC036708
• Symbole du gène: HNRNPAB
• Identification du gène (NCBI): 3182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).

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