Anticorps Polyclonal de lapin anti-HNRNPC

• Phare
• Validé par KD/KO

HNRNPC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 11760-1-AP

Synonymes

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC

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Galerie de données de validation

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  WB analysis of HeLa using 11760-1-AP

  WB result of HNRNPC antibody (11760-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPC transfected HeLa cells.

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  WB analysis using 11760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11760-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11760-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11760-1-AP

  IP result of anti-HNRNPC(IP:11760-1-AP, 4ug; Detection:11760-1-AP 1:10000) with HeLa cells lysate 1360 ug.

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  IHC staining of human lymphoma using 11760-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11760-1-AP (HNRNPC antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse colon using 11760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (11760-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 11760-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HNRNPC antibody (11760-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de côlon de souris, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 17 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11760-1-AP cible HNRNPC dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPC Protéine recombinante Ag2356
• Nom complet: heterogeneous nuclear ribonucleoprotein C (C1/C2)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa, 36-40 kDa
• Numéro d’acquisition GenBank: BC003394
• Symbole du gène: HNRNPC
• Identification du gène (NCBI): 3183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

Publications

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	WB	Nat Struct Mol Biol; Alternative polyadenylation by sequential activation of distal and proximal PolyA sites.; Authors - Peng Tang
human	WB	Adv Sci (Weinh); m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia; Authors - Zijuan Wu ; KD Validated
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Works very well in ICC (nuclear staining) but extra bands in WB

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells, SH-SY5Y cells

HNRNPC Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells, SH-SY5Y cells (Cat no:11760-1-AP)