Anticorps Monoclonal anti-HNRNPC

HNRNPC Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2G8G4

N° de cat : 68447-1-Ig

Synonymes

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC

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Galerie de données de validation

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  WB analysis using 68447-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68447-1-Ig (HNRNPC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 68447-1-Ig

  IP result of anti-HNRNPC (IP:68447-1-Ig, 4ug; Detection:68447-1-Ig 1:4000) with HeLa cells lysate 1560 ug.

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  IF Staining of HeLa using 68447-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (68447-1-Ig, Clone: 2G8G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

68447-1-Ig cible HNRNPC dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HNRNPC Protéine recombinante Ag2356
• Nom complet: heterogeneous nuclear ribonucleoprotein C (C1/C2)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC003394
• Symbole du gène: HNRNPC
• Identification du gène (NCBI): 3183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

Publications

Species	Application	Title
	IF	Oncol Res; HNRNPC as a pan-cancer biomarker and therapeutic target involved in tumor progression and immune regulation; Authors - Yuezhou Zhang

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