Anticorps Monoclonal anti-HNRNPC

HNRNPC Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G8G4

N° de cat : 68447-1-PBS

Synonymes

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC

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Galerie de données de validation

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  WB analysis using 68447-1-Ig (same clone as 68447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68447-1-Ig (HNRNPC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

  IP result of anti-HNRNPC (IP:68447-1-Ig, 4ug; Detection:68447-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (68447-1-Ig, Clone: 2G8G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68447-1-PBS cible HNRNPC dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HNRNPC Protéine recombinante Ag2356
• Nom complet: heterogeneous nuclear ribonucleoprotein C (C1/C2)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC003394
• Symbole du gène: HNRNPC
• Identification du gène (NCBI): 3183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

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