Anticorps Polyclonal de lapin anti-HNRNPF

• Phare
• Validé par KD/KO

HNRNPF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 14974-1-AP

Synonymes

Nucleolin-like protein mcs94-1, Nucleolin like protein mcs94 1, mcs94 1, HNRPF, hnRNP F

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Galerie de données de validation

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  WB analysis of HeLa using 14974-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14974-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14974-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14974-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14974-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14974-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14974-1-AP cible HNRNPF dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPF Protéine recombinante Ag6885
• Nom complet: heterogeneous nuclear ribonucleoprotein F
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC001432
• Symbole du gène: HNRNPF
• Identification du gène (NCBI): 3185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

Publications

Species	Application	Title
human	WB	Mar Drugs; An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.; Authors - Su Ching-Chyuan CC
human	WB,IF,IP	Virol J; Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication.; Authors - Aiguo Zhang ; KD Validated
human	IHC	Front Oncol; The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.; Authors - Yixin Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (06-15-2025)

Incubation ON at 4C, in 5% goat serum/PBST. Gives nuclear signal but quite weak, some cytoplasmic staining also.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa/human

HNRNPF Antibody Immunofluorescence validation (1:1000 dilution) in HeLa/human (Cat no:14974-1-AP)

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