Anticorps Monoclonal anti-HNRNPF

• Phare
• Validé par KD/KO

HNRNPF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E7A4

N° de cat : 67701-1-PBS

Synonymes

2E7A4, Heterogeneous nuclear ribonucleoprotein F, N-terminally processed, hnRNP F, HNRPF, mcs94 1

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Galerie de données de validation

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  WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

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  WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPF antibody (67701-1-Ig, Clone: 2E7A4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67701-1-PBS cible HNRNPF dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HNRNPF Protéine recombinante Ag7128
• Nom complet: heterogeneous nuclear ribonucleoprotein F
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC001432
• Symbole du gène: HNRNPF
• Identification du gène (NCBI): 3185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

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