Anticorps Polyclonal de lapin anti-HNRNPH1

HNRNPH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(14)

Avis (1)

N° de cat : 14774-1-AP

Synonymes

Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH, HNRPH1

---

Galerie de données de validation

• 

  WB analysis using 14774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14774-1-AP (HNRNPH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryonic stem cells using 14774-1-AP

  WB result from Dr. Corbit Kevin. Mouse embryonic stem cell were used to test the antibody 14774-1-AP.

• 

  IHC staining of human cervical cancer using 14774-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14774-1-AP (HNRNPH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14774-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14774-1-AP (HNRNPH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14774-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14774-1-AP (HNRNPH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules souches embryonnaires de souris, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

14774-1-AP cible HNRNPH1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPH1 Protéine recombinante Ag6506
• Nom complet: heterogeneous nuclear ribonucleoprotein H1 (H)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49-50 kDa
• Numéro d’acquisition GenBank: BC001348
• Symbole du gène: HNRNPH1
• Identification du gène (NCBI): 3187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	Nucleic Acids Res; Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.; Authors - Ruibing Chen
mouse	WB	FASEB J; 5' UTR variants in the quantitative trait gene Hnrnph1 support reduced 5' UTR usage and hnRNP H protein as a molecular mechanism underlying reduced methamphetamine sensitivity.; Authors - Qiu T Ruan
mouse	IF	Biomedicines; C9orf72 Hexanucleotide Repeat Expansion-Related Neuropathology Is Attenuated by Nasal Rifampicin in Mice.; Authors - Yukari Hatanaka

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tatyana (Verified Customer) (12-22-2024)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Nuclear staining - acceptable (a bit on a weak side). WB with semi-dry transfer 4% milk blocking in TBST, 1:1000 dilution - good signal.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa and SH-SY5Y cells/human

HNRNPH1 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in HeLa and SH-SY5Y cells/human (Cat no:14774-1-AP)

{{ptg:RelatedPrimaryAntibodies}}