Anticorps Monoclonal anti-HNRNPH1

HNRNPH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1A6C2

N° de cat : 67375-1-Ig

Synonymes

HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed

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Galerie de données de validation

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  WB analysis using 67375-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67375-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67375-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67375-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67375-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67375-1-Ig (HNRNPH1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

67375-1-Ig cible HNRNPH1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HNRNPH1 Protéine recombinante Ag6603
• Nom complet: heterogeneous nuclear ribonucleoprotein H1 (H)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49-50 kDa
• Numéro d’acquisition GenBank: BC001348
• Symbole du gène: HNRNPH1
• Identification du gène (NCBI): 3187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

Publications

Species	Application	Title
human	WB	Front Oncol; MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing; Authors - Tamara Vital
human	WB	J Cell Physiol; MACC1 enhances an oncogenic RNA splicing of IRAK1 through interacting with HNRNPH1 in lung adenocarcinoma; Authors - Shiqing Wang
human	WB,IF	Int J Biol Macromol; Japanese encephalitis virus NS3 captures the protein translation element by interacting with HNRNPH1 to promote viral replication; Authors - Xingya Wang ; KD Validated

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