Anticorps Monoclonal anti-HNRNPK

HNRNPK Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1A10C8

N° de cat : 67708-1-Ig

Synonymes

CSBP, FLJ41122, hnRNP K, HNRNPK, HNRPK, TUNP

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Galerie de données de validation

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  WB analysis using 67708-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67708-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67708-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67708-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67708-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67708-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules HuH-7, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67708-1-Ig cible HNRNPK dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HNRNPK Protéine recombinante Ag30093
• Nom complet: heterogeneous nuclear ribonucleoprotein K
• Masse moléculaire calculée: 463 aa, 51 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC014980
• Symbole du gène: HNRNPK
• Identification du gène (NCBI): 3190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability

Publications

Species	Application	Title
human	WB	Nat Commun; Autophagy-independent role of ATG9A vesicles as carriers for galectin-9 secretion; Authors - Wenting Zhang
		Cell Rep Med; Integrative proteomic profiling of tumor and plasma extracellular vesicles identifies a diagnostic biomarker panel for colorectal cancer; Authors - Jun Wang

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