Anticorps Polyclonal de lapin anti-HNRNPL

• Phare
• Validé par KD/KO

HNRNPL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18354-1-PBS

Synonymes

P/OKcl.14, HNRPL, hnRNP L

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Galerie de données de validation

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  WB analysis using 18354-1-AP (same clone as 18354-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18354-1-AP (same clone as 18354-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18354-1-AP (HNRNPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IP experiment of BxPC-3 using 18354-1-AP (same clone as 18354-1-PBS)

  IP result of anti-HNRNPL (IP:18354-1-AP, 4ug; Detection:18354-1-AP 1:4000) with BxPC-3 cells lysate 2080 ug. This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18354-1-AP (HNRNPL antibody) (right) at dilution of 1:200 compared to a negative control without primary antibody (left) (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18354-1-AP (same clone as 18354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18354-1-AP (HNRNPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18354-1-AP (same clone as 18354-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPL antibody (18354-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 18354-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18354-1-PBS cible HNRNPL dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPL Protéine recombinante Ag13211
• Nom complet: heterogeneous nuclear ribonucleoprotein L
• Masse moléculaire calculée: 572 aa, 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC069184
• Symbole du gène: HNRNPL
• Identification du gène (NCBI): 3191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.

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