Anticorps Recombinant de lapin anti-HNRNPL

HNRNPL Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3J22

N° de cat : 82259-1-PBS

Synonymes

P/OKcl.14, HNRPL, hnRNP L, 3J22

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Galerie de données de validation

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  WB analysis using 82259-1-RR (same clone as 82259-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82259-1-RR (HNRNPL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82259-1-RR (same clone as 82259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82259-1-RR (same clone as 82259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 82259-1-RR (same clone as 82259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 82259-1-RR (same clone as 82259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82259-1-RR (same clone as 82259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82259-1-RR (same clone as 82259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82259-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82259-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82259-1-RR against Human HNRNPL were performed. The affinity constant is 6.54 nM.

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Informations sur le produit

82259-1-PBS cible HNRNPL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HNRNPL Protéine recombinante Ag13211
• Nom complet: heterogeneous nuclear ribonucleoprotein L
• Masse moléculaire calculée: 572 aa, 62 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC069184
• Symbole du gène: HNRNPL
• Identification du gène (NCBI): 3191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.

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