Anticorps Polyclonal de lapin anti-HNRNPM

• Phare
• Validé par KD/KO

HNRNPM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 26897-1-AP

Synonymes

hnRNP M, HNRNPM4, HNRPM, HNRPM4, HTGR1

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Galerie de données de validation

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  WB analysis of COLO 320 using 26897-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26897-1-AP (HNRNPM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26897-1-AP

  WB result of HNRNPM antibody (26897-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPM transfected MCF-7 cells.

• 

  WB analysis using 26897-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26897-1-AP (HNRNPM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293T using 26897-1-AP

  IP and Co-IP result of anti-HNRNPM (IP:26897-1-AP, 4ug); Detection antibodies: HNRNPM 26897-1-AP 1:3000 and HNRNPC 11760-1-AP 1:3000) with HEK-293T cells lysate 1840 ug.

• 

  IHC staining of human tonsillitis using 26897-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26897-1-AP (HNRNPM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26897-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26897-1-AP (HNRNPM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26897-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 26897-1-AP (HNRNPM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26897-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPM antibody (26897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 26897-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPM antibody (26897-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules MCF-7, cellules Neuro-2a, cellules NIH/3T3
• Résultats positifs en IP: cellules HEK-293T,
• Résultats positifs en IHC: tissu d'amygdalite humain, human colon; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

26897-1-AP cible HNRNPM dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRNPM Protéine recombinante Ag25474
• Nom complet: heterogeneous nuclear ribonucleoprotein M
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 73-77 kDa
• Numéro d’acquisition GenBank: BC000138
• Symbole du gène: HNRNPM
• Identification du gène (NCBI): 4670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRNPM, also named as NAGR1, is a 730 amino acid protein, which localizes in nucleus. HNRNPM as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. HNRNPM is Involved in splicing. HNRNPM acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. HNRNPM exists two isoforms with MV 77 kDa and 73 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li ; KD Validated
human	WB	Proc Natl Acad Sci U S A; CircURI1 interacts with hnRNPM to inhibit metastasis by modulating alternative splicing in gastric cancer.; Authors - Xiaolin Wang
human	WB	J Exp Clin Cancer Res; PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.; Authors - Pingfu Hou
human	IF	Nat Commun; Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules; Authors - Tao Wang
human	WB,IP	Sci Rep; Unraveling the independent role of METTL3 in m6A modification and tumor progression in esophageal squamous cell carcinoma; Authors - Bin Du

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-22-2024)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa/human

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