Anticorps Polyclonal de lapin anti-HNRPLL

• Phare
• Validé par KD/KO

HNRPLL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 26769-1-AP

Synonymes

Stromal RNA-regulating factor, SRRF, hnRNPLL, Heterogeneous nuclear ribonucleoprotein L-like

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Galerie de données de validation

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  WB analysis using 26769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26769-1-AP (HNRPLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26769-1-AP (HNRPLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26769-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRPLL antibody (26769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

26769-1-AP cible HNRPLL dans les applications de WB, IHC, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HNRPLL Protéine recombinante Ag25287
• Nom complet: heterogeneous nuclear ribonucleoprotein L-like
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC017480
• Symbole du gène: HNRPLL
• Identification du gène (NCBI): 92906
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HNRPLL, also named as HNRNPLL and SRRF, is an RNA binding protein that regulates alternative splicing of pre-mRNA. HnRNPLL is up-regulated in stimulated T cells, bound CD45 transcripts, and is a key inducible regulator of CD45 alternative splicing (PMID: 18669861). HNRPLL has 5 isoforms with the molecular weights of 31-60 kDa. Additionally, studies have shown that HNRPLL is a metastasis suppressor gene in colorectal cancer (PMID: 28360095).

Publications

Species	Application	Title
human	WB	Stem Cell Rev Rep; Lnc-PPP2R1B Mediates the Alternative Splicing of PPP2R1B by Interacting and Stabilizing HNRNPLL and Promotes Osteogenesis of MSCs; Authors - Shuping Peng
human	WB,IHC,IF	J Exp Clin Cancer Res; Energy stress-induced circZFR enhances oxidative phosphorylation in lung adenocarcinoma via regulating alternative splicing; Authors - Zhifei Ma ; KD Validated
human	RIP,IHC	Cancer Lett; hnRNPLL regulates MYOF alternative splicing and correlates with early metastasis in pancreatic ductal adenocarcinoma; Authors - Xianghan Chen ; KD Validated

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