Anticorps Monoclonal anti-HO-1/HMOX1

• Phare
• Validé par KD/KO

HO-1/HMOX1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(174)

CloneNo.

2D10A5

Avis (5)

N° de cat : 66743-1-Ig

Synonymes

HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

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Galerie de données de validation

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  WB analysis using 66743-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66743-1-Ig

  WB result of HO-1/HMOX1 antibody (66743-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1/HMOX1 transfected HeLa cells.

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  WB analysis using 66743-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66743-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66743-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66743-1-Ig (HO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66743-1-Ig (HO-1/HMOX1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66743-1-Ig (HO-1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66743-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66743-1-Ig (HO-1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using 66743-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse spleen using 66743-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (66743-1-Ig, Clone: 2D10A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 66743-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug HO-1/HMOX1 Monoclonal antibody (66743-1-Ig, Clone:2D10A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, tissu hépatique de lapin, tissu hépatique de porc, tissu hépatique de rat, tissu splénique de porc, tissu splénique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de carcinome à cellules rénales humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris, tissu hépatique de rat
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 158 publications below
• IHC: See 14 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

66743-1-Ig cible HO-1/HMOX1 dans les applications de WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HO-1/HMOX1 Protéine recombinante Ag21296
• Nom complet: heme oxygenase (decycling) 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001491
• Symbole du gène: HO-1
• Identification du gène (NCBI): 3162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Publications

Species	Application	Title
mouse	WB	Small; Polymer-Encapsulated Catalase for Targeted Redox Regulation in Acute Liver Injury; Authors - Feifei Li
human		Dev Cell; HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis; Authors - Xiang Peng
	WB	Adv Healthc Mater; Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing.; Authors - Qiuyue Ding
mouse	WB	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
human	WB,IF	J Nanobiotechnology; Real-world of Limosilactobacillus reuteri in mitigation of acute experimental colitis; Authors - Ningning Yue
mouse	WB	Clin Immunol; Potential therapeutic effect of dimethyl fumarate on Treg/Th17 cell imbalance in biliary atresia; Authors - Mengting Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (07-24-2024)

great antibody. Incubation with primary antibody (1:3000) ON at 4C. Incubation with secondary antibody (mouse) at RT for 2h.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human cell lines

HO-1/HMOX1 Antibody Western Blot validation (1:1000 dilution) in Human cell lines (Cat no:66743-1-Ig)

FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Does not work. Non-specific band at 70 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Heart Tissue

HO-1/HMOX1 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:66743-1-Ig)

FH

Ayse Tarbin (Verified Customer) (03-16-2023)

It was a gift antibody. It worked perfectly after 2h incubation at room temp. Definately I will consider to buy it.

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:2000 in 5% milk in TBST
• Cell Tissue Type: Mouse satellite cells

HO-1/HMOX1 Antibody Western Blot,Cell culture validation (1:2000 in 5% milk in TBST dilution) in Mouse satellite cells (Cat no:66743-1-Ig)

FH

Hala (Verified Customer) (08-17-2021)

HO-1 expression was revealed with a donkey anti-mouse 547 red secondary antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: hepG2

HO-1/HMOX1 Antibody Immunofluorescence validation (1/100 dilution) in hepG2 (Cat no:66743-1-Ig)

FH

Marta (Verified Customer) (09-29-2020)

Western blotting of Hmox1 in mouse liver. Used at a 1:1000 dilution in 3% milk and TBST overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

HO-1/HMOX1 Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:66743-1-Ig)

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