Anticorps Recombinant de lapin anti-HO-1

• Phare
• Validé par KD/KO

HO-1 Recombinant Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2M6

N° de cat : 81281-1-PBS

Synonymes

HMOX1, HO-1/HMOX1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

---

Galerie de données de validation

• 

  WB analysis using 81281-1-RR (same clone as 81281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

  WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 81281-1-RR (same clone as 81281-1-PBS)

  IP result of anti-HO-1 (IP:81281-1-RR, 4ug; Detection:81281-1-RR 1:8000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 81281-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81281-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81281-1-RR against Human HO-1 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

81281-1-PBS cible HO-1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HO-1 Protéine recombinante Ag1190
• Nom complet: heme oxygenase (decycling) 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001491
• Symbole du gène: HO-1
• Identification du gène (NCBI): 3162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).

{{ptg:RelatedPrimaryAntibodies}}