Anticorps Recombinant de lapin anti-HO-1/Hmox1

HO-1/Hmox1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240236G6

N° de cat : 83291-2-PBS

Synonymes

Hmox1, HO-1, D8Wsu38e, heme oxygenase (decycling) 1, Hemox

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00318-3

  Sandwich ELISA standard curve of MP00318-3, mouse HO-1/Hmox Recombinant Matched Antibody Pair - PBS only. 83291-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0938. 83291-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00318-3

  Serum of sixteen mice was measured. The mouse HO-1/Hmox1 concentration of detected samples was determined to be 547.1 pg/mL with a range of 297.1 - 1,158.6 pg/mL.

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  Sample test of MP00318-3

  Mouse spleen tissue (3 spleen in 1-2 mm pieces in 100 mL of medium) was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse HO-1/Hmox1,and measured 225.2 pg/mL.

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  Sample test of MP00318-3

  The mean mouse HO-1/Hmox1 concentration was determined to be 23,262.0 pg/mL in NIH/3T3 cell extract based on a 7.8 mg/mL extract load and 41,977.1 pg/mL in mouse liver extract based on a 5 mg/mL extract load.

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Informations sur le produit

83291-2-PBS cible HO-1/Hmox1 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HO-1/Hmox1 Protéine recombinante Eg0938
• Nom complet: heme oxygenase (decycling) 1
• Masse moléculaire calculée: 33 kDa
• Numéro d’acquisition GenBank: NM_010442.2
• Symbole du gène: HO-1
• Identification du gène (NCBI): 15368
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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