Anticorps Recombinant de lapin anti-HO-1/Hmox1

HO-1/Hmox1 Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240236G4

N° de cat : 83291-3-PBS

Synonymes

Hmox1, HO-1, 240236G4, D8Wsu38e, heme oxygenase (decycling) 1

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Galerie de données de validation

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  WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 83291-3-RR (same clone as 83291-3-PBS)

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug HO-1/Hmox1 Recombinant antibody (83291-3-RR, Clone:240236G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83291-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83291-3-RR against Mouse HO-1/Hmox1 were performed. The affinity constant is 1.23 nM.

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Informations sur le produit

83291-3-PBS cible HO-1/Hmox1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HO-1/Hmox1 Protéine recombinante Eg0938
• Nom complet: heme oxygenase (decycling) 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: NM_010442.2
• Symbole du gène: HO-1
• Identification du gène (NCBI): 15368
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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