Anticorps Monoclonal anti-HOMER1

HOMER1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B1B11

N° de cat : 68597-1-PBS

Synonymes

3B1B11, HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1

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Galerie de données de validation

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  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68597-1-Ig (same clone as 68597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68597-1-Ig (HOMER1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 68597-1-Ig (same clone as 68597-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using HOMER1 antibody (68597-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68597-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68597-1-PBS cible HOMER1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HOMER1 Protéine recombinante Ag21302
• Nom complet: homer homolog 1 (Drosophila)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40-46 kDa
• Numéro d’acquisition GenBank: BC015502
• Symbole du gène: HOMER1
• Identification du gène (NCBI): 9456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

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