Anticorps Recombinant de lapin anti-HOMER1

HOMER1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243007G2

N° de cat : 85692-4-PBS

Synonymes

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1

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Galerie de données de validation

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  WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IF Staining of MOLT-4 using 85692-4-RR (same clone as 85692-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using HOMER1 antibody (85692-4-RR, Clone: 243007G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85692-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85692-4-RR against Human HOMER1 were performed. The affinity constant is 0.309 nM.

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Informations sur le produit

85692-4-PBS cible HOMER1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HOMER1 Protéine recombinante Ag3109
• Nom complet: homer homolog 1 (Drosophila)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 46-48 kDa
• Numéro d’acquisition GenBank: BC015502
• Symbole du gène: HOMER1
• Identification du gène (NCBI): 9456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

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